Temporal expression profiling of novel Spodoptera litura nucleopolyhedrovirus-encoded microRNAs upon infection of Sf21 cells
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چکیده
منابع مشابه
Transcriptome Analysis of the SL221 Cells at the Early Stage during Spodoptera litura Nucleopolyhedrovirus Infection
Spodoptera litura (S. litura) is one of the most destructive agricultural pests worldwide. There is urgent need for a nuclear polyhedrosis virus that is specific to S. litura. To date, there have been no reports regarding the responses of S. litura cells to early Spodoptera litura nucleopolyhedrovirus (SpltNPV) infection due to the lack of a reference genome and transcriptome for S. litura. In ...
متن کاملApoptosis is induced in the haemolymph and fat body of Spodoptera exigua larvae upon oral inoculation with Spodoptera litura nucleopolyhedrovirus.
Spodoptera exigua multinucleopolyhedrovirus (SeMNPV) and Spodoptera litura nucleopolyhedrovirus (SpltNPV) are genetically similar, but the larvae of S. exigua are not susceptible to SpltNPV. The aim of this study was to identify whether any process was inhibiting SpltNPV infection at some point. S. exigua larvae infected with a high concentration of wild-type SpltNPV by oral inoculation produce...
متن کاملIdentification, Expression and Target Gene Analyses of MicroRNAs in Spodoptera litura
MicroRNAs (miRNAs) are small RNAs widely present in animals and plants and involved in post-transcriptional regulation of gene transcripts. In this study we identified and validated 58 miRNAs from an EST dataset of Spodoptera litura based on the computational and experimental analysis of sequence conservation and secondary structure of miRNA by comparing the miRNA sequences in the miRbase. RT-P...
متن کاملEfficacy of Spodoptera litura multiple nucleopolyhedrovirus after serial passage through the homologous insect larval host.
An originally isolated baculovirus, Spodoptera litura multiple nucleopolyhedrovirus (SpltMNPV) was serially passed through the S. litura larvae for upto four generations to determine the mean number of occlusion bodies (OBs) harvested per larva and their efficacy in terms of infectivity, feeding cessation and speed of kill of host larvae. The results revealed that the mean number of OBs harvest...
متن کاملIdentification and Characteristics of microRNAs from Army Worm, Spodoptera frugiperda Cell Line Sf21
microRNAs play important regulatory role in all intrinsic cellular functions. Amongst lepidopteran insects, miRNAs from only Bombyx mori have been studied extensively with a little focus on Spodoptera sp. In the present study, we identified a total of 226 miRNAs from Spodoptera frugiperda cell line Sf21. Of the total, 116 miRNAs were well conserved within other insects, like B. mori, Drosophila...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 2015
ISSN: 0022-1317,1465-2099
DOI: 10.1099/jgv.0.000008